Coding

Part:BBa_K4214002

Designed by: Jol Tekoniemi   Group: iGEM22_Stockholm   (2022-10-08)


hGIF with 6xHis-tag

Human GIF with His6-tag. This part has the native signal peptide removed, as it is not required for bacterial expression or in our mammalian expression vector. This part was used to produce and purify our Human GIF in bacteria, through the composite parts Ba_K421403(with LacI promotor) and BBa_K421404 (with LacI promotor and pelB leader sequence).

Fig.: Human GIF with His6-tag (BBa_K4214002)

Usage and Biology

  • NCBI page of the human gastric intrinsic factor [1]
  • This part of hGIF does not contain the native signal peptide (AA1-18)
  • This part was used in parts BBa_K4214003 and BBa_K4214004

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 257
    Illegal BsaI.rc site found at 937
    Illegal SapI site found at 927


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Categories
Parameters
None